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human leukemic cell line jurkat-t  (China Center for Type Culture Collection)
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China Center for Type Culture Collection human leukemic cell line jurkat-t
Decarboxylation of F-5caC is a rapid process and occurs in diverse mammalian cells. (a–c) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA <t>of</t> <t>HEK293T</t> cells upon feeding of 300 μM of F-5caC for different times. (d) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of <t>Jurkat-T,</t> MCF-7, and Neuro-2a cells upon feeding of 300 μM of F-5caC for 3 d. (e) Schematic illustration of the active 5mC demethylation through two pathways: the direct decarboxylation of TET-produced 5caC to cytosine in mammalian genomes established in the current study and the previous TET-TDG-BER pathway.
Human Leukemic Cell Line Jurkat T, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human leukemic cell line jurkat-t - by Bioz Stars, 2026-09
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Decarboxylation of F-5caC is a rapid process and occurs in diverse mammalian cells. (a–c) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of HEK293T cells upon feeding of 300 μM of F-5caC for different times. (d) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of Jurkat-T, MCF-7, and Neuro-2a cells upon feeding of 300 μM of F-5caC for 3 d. (e) Schematic illustration of the active 5mC demethylation through two pathways: the direct decarboxylation of TET-produced 5caC to cytosine in mammalian genomes established in the current study and the previous TET-TDG-BER pathway.

Journal: Chemical Science

Article Title: Direct decarboxylation of ten-eleven translocation-produced 5-carboxylcytosine in mammalian genomes forms a new mechanism for active DNA demethylation †

doi: 10.1039/d1sc02161c

Figure Lengend Snippet: Decarboxylation of F-5caC is a rapid process and occurs in diverse mammalian cells. (a–c) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of HEK293T cells upon feeding of 300 μM of F-5caC for different times. (d) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of Jurkat-T, MCF-7, and Neuro-2a cells upon feeding of 300 μM of F-5caC for 3 d. (e) Schematic illustration of the active 5mC demethylation through two pathways: the direct decarboxylation of TET-produced 5caC to cytosine in mammalian genomes established in the current study and the previous TET-TDG-BER pathway.

Article Snippet: The human embryonic kidney epithelial cell line (HEK293T), human leukemic cell line (Jurkat-T), human breast adenocarcinoma cell line (MCF-7), and mouse neuroblastoma N2a cell line (Neuro-2a) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Produced